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43 kD單條蛋白Marker
貨號 |
名(ming)稱 |
規格(ge) |
RA5020M |
43 kD單條蛋白Marker |
~500次(ci) |
●產品組份:
貨號(hao) |
名(ming)稱 |
規格(ge) |
貯存 |
RA5020M-01 |
43 kD單條蛋白(bai)Marker粉(fen)末 |
1 mg |
-20℃ |
PL080-01 |
5×MonoColor蛋(dan)白上樣緩(huan)沖液(變(bian)性,還(huan)原) |
1 ml |
-20℃ |
● 儲存條件、效期及運輸:
-20℃保存;配制(zhi)后有效期一年;濕冰(bing)運輸。
● 產品簡介:
本產品包含一種已知分子量的標準蛋白質,分子量大小為43 kD,可以用來判斷 SDS-PAGE未知蛋白(bai)的分子(zi)量。43 kD單條蛋白(bai)以干粉狀態提(ti)供。
以(yi)每次上樣10 μl(2 μg)計算,本產品可以(yi)使用(yong)約500次。
● 使用說明:
1. 配制1 mg/ml 43 kD蛋白Marker母液:
粉末管中中直接加入1ml超純水,此溶液-20℃貯存。
2. 按照下表配制Marker溶液
配制量 |
濃度 |
43 kD母(mu)液 (1 mg/ml) |
超純(chun)水(shui) |
5×蛋白(bai)上樣緩(huan)沖液(ye) |
1 M DTT (可選) |
50 μl |
0.2 μg/μl |
10 μl |
30 μl |
10 μl |
2.5 μl |
100 μl |
0.2 μg/μl |
20 μl |
60 μl |
20 μl |
5 μl |
注:由(you)于蛋白(bai)上樣(yang)緩(huan)沖液中DTT含量(liang)會隨(sui)貯存時間延長(chang)而降低,可以選擇補加DTT。
3. 使用方法:
3.1 取配制好的溶液徹底混勻后,95℃處理5分(fen)鐘立即上(shang)樣(yang)電泳(yong),每次上(shang)樣(yang)10 μl。
3.2 電泳結束后,染色,觀察結果。
4. 實(shi)驗示例:
使(shi)用蛋白Marker產品發表部(bu)分文章列表:
1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.
Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang
Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)
Journal: Food Control 50 (2015) 896e900
Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University
Paper link:
2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by isolated from fermented sausage Lactobacillus plantarum.
Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie
Zhang, Changqing Yu, Xue Han
Marker: RTD6110,彩虹超低(di)分子量(liang)蛋白Marker(1.2-45 kD)
Journal: Food Control Volume 81, November 2017, Pages 211-217
Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University
Paper link:
3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.
Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu
Marker: RTD6110,彩虹超低(di)分子量蛋(dan)白Marker(1.2-45 kD)
Journal: Frontiers in Plant Science. Volume 81, November 2017, Pages 211-217
Institution:Institute of Plant Protection, Chinese Academy of Agricultural Sciences
Paper link:
4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.
Author: Xinran Lv, Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li
Marker: RTD6127,超(chao)低(di)分子量蛋白Marker IV(3.3-45 kD)
Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)
Institution:College of Biological Science and Biotechnology, Beijing Forestry University
Paper link:
5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.
Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu
Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)
Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626
Institution:Institute of Plant Protection, Chinese Academy of Agricultural Sciences
Paper link:
6. [2021 IF=11.277] An Alternative Splicing Variant of PtRD26 Delays Leaf Senescence by Regulating Multiple NAC Transcription Factors in Populus.
Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li
Marker: RTD6110,彩虹預染超低分子量蛋白(1.2-45 kD)
Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614
Institution:Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University
Paper link:
7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Author: Feng Qin, Shujuan Wang, Mingxia Gao and Xiangmin Zhang
Marker: RTD6110,彩虹預(yu)染超低分子量(liang)蛋(dan)白(1.2-45 kD)
Journal: Analytical Methods. 2021,13, 2804-2811
Institution:Department of Chemistry, Fudan University
Paper link:
8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.
Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng
Marker: RTD6142 高分子量非變性電(dian)泳(yong)蛋白(bai)質(zhi)Marker II(45-669 kD)
Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892
Institution:College of Veterinary Medicine, Hunan Agricultural University,
Paper link:
9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger
Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia
Marker: RTD6109 高分子量蛋白質Marker(43-200 kD)
Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632
Institution:College of Food Science and Technology, Hebei Normal University of Science and Technology
Paper link:
10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.
Author: Dong Liu
Marker: RTD6103 低分子量蛋白質Marker II(14.4-116 kD)
Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33
Institution:Department of Food Processing Technology, Anqing Vocational & Technical College
Paper link:
11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy
Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu
Marker: Protein Marker
Journal: Biomedical Materials, 2022 Jan 24;17(2)
Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China
Paper link:
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