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首頁 > 核酸生物學 > 核酸電泳 > 雙鏈DNA Marker

DNA Marker III(300-5000bp)

DNA Marker III(300-5000bp)

產品編號:RTM403-02

產品規格:100次

數量
價格 ¥150

精準(zhun)定量

Marker III DNA Ladder

 

● 產品編號及規格:

RTM403-02       100T (2×250μl)        150元

● 產品簡介:

本產品(pin)是(shi)由7條帶狀雙鏈(lian)DNA條(tiao)帶組成,適用(yong)于瓊脂糖凝(ning)膠電泳(yong)中(zhong)DNA條帶(dai)的分析。產品中已(yi)含有1×loading buffer,可根(gen)據實(shi)驗需要,直接(jie)取2-5μl電泳,使用方便,電泳圖像清晰。7條帶的大(da)小分別為3005008001500200030005000bp。其中(zhong)1500bp條帶(dai)含(han)量為100ng/5μl,顯示加亮(liang)帶(dai),其余(yu)條帶(dai)含量為(wei)50ng/5μl


● 儲存條件:

  4 貯存(cun)6個月(-20貯(zhu)存一年)。

● 使用方法:

1. 2-5μl本產品加入到瓊(qiong)脂糖凝膠(jiao)的加樣孔中就(jiu)行電泳。

注(zhu):根據梳子的厚(hou)度和寬(kuan)度進(jin)行上樣(yang)。每1mm×1mm(厚(hou)度×寬(kuan)度)加樣孔上樣1μl。窄齒梳子(一般(ban)為1mm×2mm)上樣2μl;寬齒梳子(一(yi)般為1mm×5mm)上(shang)樣(yang)5μl。如果使用厚齒梳子或寬于5mm的梳子,可以適當(dang)調整上樣量

2. 建議電(dian)泳條件:凝膠濃(nong)度為1.0%,凝膠長度(du)5-7cm,電泳電壓(ya)4-10v/cm,電泳(yong)時(shi)間(jian)25-30分鐘。

3. 通過EB染色后紫外(wai)燈(deng)下觀察條帶。

注:如果使用Goldview等染(ran)料就行(xing)染(ran)色,由(you)于靈(ling)敏度比EB低(di),請(qing)酌情增加(jia)Marker的上樣量;如果(guo)使(shi)用SYBR類染(ran)料染(ran)色,由于(yu)靈(ling)敏度比EB高,請酌情降低Marker的(de)上樣(yang)量

● 注意事項(xiang):

1. 瓊(qiong)脂(zhi)糖(tang)的(de)質量對DNA的(de)電(dian)泳(yong)有很大影響,電(dian)泳(yong)時請盡量使用質量優等的(de)瓊脂(zhi)糖(tang)。

2. 請(qing)使(shi)用(yong)新鮮配制的(de)電泳(yong)(yong)緩沖液和新鮮配制的(de)瓊脂糖凝膠進(jin)行電泳(yong)(yong),以(yi)保(bao)證(zheng)Marker良好的分離效果。

3. Marker適用(yong)于DNA片(pian)段大小的(de)確(que)定(ding)和對DNA含(han)量的精確定量。


使用DNA Marker產品發表部分文章列表:

 1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415,D2000 DNA ladder

Journal: Phytotaxa. 2021,483 (2): 085–094.

Institution:University of Electronic Science and Technology of China

Paper link

 

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403,DNA Marker III

Author: ZihanSong,ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution Zhejiang University

Paper link

 

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404,DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos oneJanuary 2012 | Volume 7 | Issue 1 | e29875

InstitutionState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link

 

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409,100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos oneSeptember 2013 | Volume 8 | Issue 9 | e737

InstitutionSchool of Agriculture and Biology, Shanghai Jiao Tong University

Paper link

 

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409,100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu,Z.-L. Zhang

Journal: Plant Biology14 (2012) 828–838

InstitutionKey Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper link

 

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415,D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos OnePublished: December 21, 2015

InstitutionKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link

 

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415,D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER,ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa2015,227 (3): 229–242

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link

 

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415,D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link

 

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421,λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

InstitutionKey Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link

 

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415,D2000 DNA ladder

Author: Huan Jiang,Wenxing Long, Hui Zhang,Chengneng Mi,Tao Zhou,Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

InstitutionInstitute of Tropical Agriculture and Forestry, Hainan University

Paper link 

 

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406,DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

InstitutionState Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link 

 

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415,D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7,2021,3071-3077

InstitutionBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link

 

 

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415,D2000 DNA ladder

Author: Kevin D Hyde,Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

InstitutionSchool of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link



 
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