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精準定量DNA Marker VI
● 產(chan)品編號(hao)及規格(ge):
RTM406-02 100T (2×250μl)
● 產品簡介:
本(ben)產品(pin)是由6條(tiao)帶狀(zhuang)雙鏈DNA條帶組成,適用于瓊脂糖(tang)凝膠(jiao)電泳中DNA條帶的(de)分析。產(chan)品中已含有1×loading buffer,可(ke)根據實(shi)驗需(xu)要,直接取2-5μl電泳(yong),使(shi)用(yong)方便(bian),電泳(yong)圖像清晰。6條帶的(de)大小分別為250,1000,2500,5000,7000,10000bp。其中2500bp條帶(dai)含量為100ng/5μl,顯(xian)示加(jia)亮帶,其余條帶濃度為50ng/5μl。
● 儲存條件:
4℃ 貯存6個月(-20℃貯存一年)。
● 使用方法:
1. 取(qu)2-5μl本產(chan)品加入到瓊脂糖凝膠的加樣孔中就行電泳。
注(zhu):根(gen)據電泳梳子(zi)的厚度和(he)寬(kuan)度確定上樣量。每1mm×1mm(厚(hou)度×寬度)加樣孔(kong)上樣1μl;窄(zhai)齒(chi)梳子(一般(ban)為1mm×2mm)上樣(yang)2μl;寬齒梳子(一(yi)般為1mm×5mm)上樣5μl。如果使用厚齒梳子(zi)或寬(kuan)于5mm的(de)梳子,可以適當調整(zheng)上樣量。
2. 建議電泳條件:凝膠濃度為1.0%,凝膠長度5-7cm,電泳(yong)電壓4-10v/cm,電泳時間(jian)25-30分鐘。
3. 通(tong)過EB染色后紫外燈下觀察條帶。
注:如果使用Goldview等(deng)染料就行(xing)染色,由(you)于靈敏度(du)比(bi)EB低,請酌情增加Marker的上(shang)樣量。
● 注意事項:
1. 瓊脂糖的(de)質量對DNA的(de)電(dian)泳有很大影響,電(dian)泳時請盡量使用質量優(you)等(deng)的(de)瓊脂糖。
2. 請使(shi)用(yong)新(xin)鮮配(pei)制的電泳緩(huan)沖液和新(xin)鮮配(pei)制的瓊脂糖凝膠進行電泳,以保證Marker良好的分離效果。
3. 該Marker適用于(yu)DNA片段大小的確定和對DNA含(han)量(liang)的精確定量(liang)。使用DNA Marker產(chan)品發(fa)表部分文章列(lie)表:
1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.
Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI
Marker: RTM415,D2000 DNA ladder
Journal: Phytotaxa. 2021,483 (2): 085–094.
Institution:University of Electronic Science and Technology of China
Paper link:
2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.
Marker :RTM403,DNA Marker III
Author: ZihanSong,ShengCai.
Journal: Talanta. Vol 239, 2022, 123129
Institution: Zhejiang University
Paper link:
3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.
Marker :RTM404,DNA Marker IV
Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang
Journal: PLos one,January 2012 | Volume 7 | Issue 1 | e29875
Institution:State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology
Paper link:
4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.
Marker :RTM409,100bp DNA ladder
Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen
Journal: PLos one,September 2013 | Volume 8 | Issue 9 | e737
Institution:School of Agriculture and Biology, Shanghai Jiao Tong University
Paper link:
5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.
Marker :RTM409,100bp DNA ladder
Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu,Z.-L. Zhang
Journal: Plant Biology,14 (2012) 828–838
Institution:Key Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences
Paper link:
6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).
Marker :RTM415,D2000 DNA ladder
Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang
Journal: Plos One,Published: December 21, 2015
Institution:Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Paper link:
7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.
Marker :RTM415,D2000 DNA ladder
Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER,ALI H.BAHKALI, KEVIN D. HYDE
Journal: Phytotaxa,2015,227 (3): 229–242
Institution:State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences
Paper link:
8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)
Marker :RTM415,D2000 DNA ladder
Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde
Journal: Fungal Diversity (2012) 56:157–171
Institution:State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences
Paper link:
9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.
Marker :RTM421,λ DNA/HindIII
Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin
Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li
Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30
Institution:Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal
University
Paper link:
10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.
Marker :RTM415,D2000 DNA ladder
Author: Huan Jiang,Wenxing Long, Hui Zhang,Chengneng Mi,Tao Zhou,Zongzhu Chen
Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30
Institution:Institute of Tropical Agriculture and Forestry, Hainan University
Paper link:
11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.
Marker :RTM406,DNA Marker VI
Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,
Ida Bagus Andika, Hideki Kondo and Liying Sun
Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076
Institution:State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University
Paper link:
12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.
Marker :RTM415,D2000 DNA ladder
Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang
Journal: Journal of the Science of Food and Agriculture Volume101, Issue7,2021,3071-3077
Institution:Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences
Paper link:
13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)
Marker :RTM415,D2000 DNA ladder
Author: Kevin D Hyde,Jian-Kui Liu
Journal: Fungal Diversity volume 112, pages 1–88 (2022)
Institution:School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China
Paper link:
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