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RNAplant
植物RNA提取試劑
產(chǎn)品簡介:
RNAplant試劑可從植物組織,特別是富含多酚或淀粉的植物組織中(如馬鈴薯塊莖,白松松針或嫩苗,和西紅柿葉等)提取高純度的總RNA。
每100 ml可處理100 mg組織200次。
保存條件:室溫運(yùn)輸,4℃保存,可保存6-12個(gè)月。
準(zhǔn)備試劑:液氮,研缽,無RNase離心管,5 M NaCl(NC1050F 5M 氯化鈉 RNase-free,DNase-free),氯仿,異丙醇,75%乙醇(無RNase水配制),無RNase水。
小規(guī)模提取操作步驟:(樣品<0.1 g ,使用小規(guī)模提取方法):
1. 取不多于0.1 g植物組織,在液氮中研碎,轉(zhuǎn)移到1.5 ml 離心管中,加0.5 ml提取試劑,振蕩至徹底混勻。
注:振蕩不要太劇烈,否則容易導(dǎo)致基因組DNA污染。
2. 室溫放置5分鐘。
3. 4℃ 12, 000 rpm離心2-3分鐘,上清轉(zhuǎn)入新的無RNase離心管。
4. 上清中加入0.1 ml 5 M NaCl(NC1050F 5M 氯化鈉 RNase-free,DNase-free),溫和混勻。
5. 加入0.3 ml 氯仿,上下顛倒混勻。
6. 4℃ 12,000 rpm離心10-15分鐘,取上層水相轉(zhuǎn)入新的無RNase離心管。
7. 加與所得水相等體積的異丙醇,混勻,室溫放置10分鐘。
8. 4℃ 12,000 rpm離心10-15分鐘。棄掉上清,注意不要倒出沉淀。加1 ml 75%乙醇沖洗沉淀。(沉淀可能很難看見,應(yīng)小心操作。)
9. 4℃ 5000-7000rpm離心3分鐘。倒出液體,注意不要倒出沉淀。剩余的少量液體短暫離心,用槍頭吸出,室溫晾干1-2分鐘至沉淀干燥。
10. 加適量無RNase水充分溶解RNA,-70℃保存。
注意事項(xiàng):
RNAplant有刺激性味道,操作時(shí)請(qǐng)?jiān)谕L(fēng)處進(jìn)行。RNAplant植物RNA提取試劑(多糖多酚植物)(貨號(hào):RTR2303)發(fā)表文章列表(15篇):
1. [2021 IF=4.7] The 5-untranslated region of the FAD3 mRNA is required for its translational enhancement at low temperature in Arabidopsis roots.
Author: Chun-TaoWang, Yi-Nong Xu.
Journal: Plant Science 179,234-240,2010
植物材料:擬南芥
Institution: Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences,
Paper link:https://linkinghub.elsevier.com/retrieve/pii/S0168945210001470
2. [2021 IF=4.1] Different exogenous sugars affect the hormone signal pathway and sugar metabolism in ‘‘Red Globe’’ (Vitis vinifera L.) plantlets grown in vitro as shown by transcriptomic analysis.
Author: Juan Mao,Wenfang Li,Baoqin Mi,Zonghuan Ma,Yongmei Zhang, Baihong Chen
Journal: Planta 246, 537–552,2017
植物材料:葡萄
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s00425-017-2712-x
3. [2021 IF=3.4] Transcriptome analysis revealed glucose application afects plant hormone signal transduction pathway in “Red Globe” grape plantlets s.
Author: Juan Mao ,Wenfang Li, Baoqin Mi, Zonghuan Ma, Mohammed Mujitaba Dawuda, Cunwu Zuo
Journal: Plant Growth Regul volume 84, pages 45–56 (2018)
植物材料:葡萄
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s10725-017-0320-1
4. [2021 IF=4.169] Effects of CEPA and 1-MCP on flower bud differentiation of apple cv. 1 agafu No.2 grafted on different rootstocks.
Author: Wenfang Li, Baihong Chen
Journal: J Plant Growth Regul. volume 38, pages 842–854 (2019)
植物材料:蘋果
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://link.springer.com/article/10.1007/s00344-018-9895-7
5. [2021 IF=7.228] FIT and bHLH Ib transcription factors modulate iron and copper crosstalk in Arabidopsis.
Author: Yuerong Cai, Yang Li, Gang Liang
Journal: Plant, Cell and Environment. Volume44, Issue5 May 2021 Pages 1679-1691
植物材料:擬南芥
Institution: CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden
Paper link:https://onlinelibrary.wiley.com/doi/10.1111/pce.14000
6. [2022 IF=6.2] Genome-Wide Identification and Abiotic Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries.
Author: Lili Guo, Shixiong Lu, Tao Liu, Guojie Nai, Jiaxuan Ren, Huimin Gou, Baihong Chen,Juan Mao
Journal: International Journal of Molecular Sciences. 2023, 24, 4049.
植物材料:草莓
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.3390/ijms24044049
7. [2022 IF=2.561] Physiological and Transcriptomic Analysis Reveals Drought?Stress Responses of Arabidopsis DREB1A in Transgenic Potato
Author: Xiaoxia Jia, Enfang Qi, Shi Liu, Sheng Ma, Guohong Wen, Xinhui Zhang, Heping Lv, Wei Huang, Xucheng Zhang
Journal: Potato Research Published online 21 Feb 2023
植物材料:馬鈴薯
Institution: Gansu Academy of Agricultural Sciences
Paper link:https://link.springer.com/article/10.1007/s11540-023-09619-7
8. [2022 IF=6.6] Molecular evolution and expression assessment of DFRs in apple
Author: Wen?Fang Li, Ju Gao, Zong?Huan Ma, Ying?Jun Hou, Xin Li, Juan Mao and Bai?Hong Chen
Journal: Chemical and Biological Technologies in Agriculture (2023) 10:98
植物材料:蘋果
Institution: College of Horticulture, Gansu Agricultural University
Paper link: https://doi.org/10.1186/s40538-023-00470-z
9. [2022 IF=3.5] Genome-wide identification and expression analysis of the BURP domain-containing genes in Gossypium hirsutum
Author: Jiaxuan Ren,Li Feng,Lili Guo, Huimin Gou,Shixiong Lu, Juan Mao
Journal: Physiology and Molecular Biology of Plants 2023 29(11):1717–1731
植物材料:棉花
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.1007/s12298-023-01393-7
10. [2022 IF=2.9] Genome-wide identification and expression analysis of the ZRT, IRT-like Protein (ZIP) family in Nicotiana tabacum
Author: Zhijie Duan, Deka Reine Judesse Soviguidi, Bangzhen Pan, Rihua Lei,Zhongbang Song, Gang Liang
Journal: Metallomics 2024 Oct
植物材料:煙草
Institution: Xishuangbanna Botanical Garden,CAS
Paper link:https://pubmed.ncbi.nlm.nih.gov/39390668/
11. [2023 IF=3.9] Genome Wide Identifcation and Expression Analysis of the NRL (NPH3/RPT2 Like) Family in Vitis vinifera
Author: Yubin Li,Hong Pan, Yan Hao,Yanfang Zhu, Qiang Chang, Jiaxuan Ren
Journal: Journal of Plant Growth Regulation Published online 23 Oct 2024
植物材料:葡萄
Institution: Institute of Forestry, Fruits and Floriculture, Gansu Academy of Agricultural Sciences
Paper link:https://doi.org/10.1007/s00344-024-11530-5
12. [2023 IF=6.1] Genome-wide survey of chlorophyllase (CLH) gene family in seven Rosaceae and functional characterization of MdCLH1 in apple (Malus domestica) leaf photosynthesis.
Author: Wen-Fang Li, Ji Liu, Tian-Yu Hu, Ying-Jun Hou, Zong-Huan Ma, Tong Feng, Zhi-Gang Guo, Juan Mao, Bai-Hong Chen
Journal: Plant Physiology and Biochemistry 219 (2025) 109387
植物材料:蘋果
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.1016/j.plaphy.2024.109387
13. [2023 IF=4.9] Shading Treatment Reduces Grape Sugar Content by Suppressing Photosynthesis-Antenna Protein Pathway Gene Expression in Grape Berries.
Author: Xintong Nan, Wenfang Li, Miao Shao, Zimeng Cui, Han Wang, Jiaxing Huo, Lizhen Chen, Baihong Chen,Zonghuan Ma
Journal: International Journal of Molecular Sciences 2024, 25, 5029.
植物材料:葡萄
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.3390/ijms25095029
14. [2023 IF=4.3] Genome-wide identification of potato Trihelix gene family and its response to different abiotic stresses.
Author: Hongyu Yang, Yan Wang, Taotao Liu, Wenxia Yao, Xiangjun Fan, Bin Yu,Guiying Shi.
Journal: BMC Plant Biology (2025) 25:690
植物材料:馬鈴薯
Institution: College of Horticulture, Gansu Agricultural University
Paper link:https://doi.org/10.1186/s12870-025-06437-6
15. [2025 IF=4.1] MdGRF22, a 14-3-3 Family Gene in Apple, Negatively Regulates Drought Tolerance via Modulation of Antioxidant Activity and Interaction with MdSK.
Author: Jiaxuan Ren, Hong Wang, Mingxin Zhao, Guoping Liang, Shixiong Lu,Juan Mao.
Journal: Plants 2025, 14, 1968
植物材料:蘋果
Institution: Institute of Forestry, Fruits and Floriculture, Gansu Academy of Agricultural Sciences
Paper link:https://doi.org/10.3390/plants14131968
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