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檢測到條帶，25 kD
Electrophoresis: 12% RealPAGE SDS Electrophorosis(RTD6148)
Transfer: 1×RealBlot transfer buffer(RT5020)
350mA blotting to NC membrane for 30min.
Blocking: BSA at RT for 1h.
Primary antibody: Lhca3 1:5000 dilution at RT for 1h
Secondary antibody: 1:5000 dilution using Goat Anti-Rabbit IgG AP
Detection: using BCIP/NBT at RT for 5 min