2×Taq PCR MasterMix
● 產品包裝：
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目(mu)錄號
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包裝(zhuang)
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價格
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2×MasterMix（不(bu)含染料）
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RTC3103-02
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500μl
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60元
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RTC3103-03
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5×500μl
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240元
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2×MasterMix（含染(ran)料）
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RTC3104-02
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500μl
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60元
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RTC3104-03
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5×500μl
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240元(yuan)
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注(zhu)：以50μl PCR反應體(ti)系為例，每(mei)次使用25μl 2×MasterMix，500μl可做20次 50μl PCR反應。
● 儲(chu)存條(tiao)件: -20℃可長期保存(cun)(cun)；4℃穩定貯存(cun)(cun)3個(ge)月。經常使(shi)用(yong)時，一旦融(rong)化后(hou)請4℃貯存(cun)(cun)，盡量避免反(fan)復凍融(rong)。
● 產品簡介(jie)：
本(ben)產品包(bao)含Taq DNA聚合酶(mei)、dNTPs、MgCl2、反(fan)(fan)(fan)應(ying)緩沖液、PCR反(fan)(fan)(fan)應(ying)的(de)增(zeng)強劑和優化劑以(yi)及穩定劑，濃度為2×。使用(yong)時，只(zhi)需補加實驗(yan)所需的(de)引物和模(mo)板(ban)后(hou)即可(ke)進行PCR反(fan)(fan)(fan)應(ying)，可(ke)最大(da)限度的(de)減少人為誤差，具有(you)快速(su)簡便、穩定性好(hao)等優點。適(shi)用(yong)于常規PCR反(fan)(fan)(fan)應(ying)、復雜模(mo)板(ban)（如GC含(han)量高，有(you)二(er)級(ji)結構）的(de)擴增(zeng)以(yi)及大(da)規模(mo)基因檢測。使用(yong)該產品(pin)得到(dao)的(de)PCR擴增(zeng)產物末端含(han)有(you)一個A堿基，可(ke)以(yi)直接用(yong)于TA克隆。
● 質(zhi)量控制：
經檢測無外源(yuan)核酸酶活性；PCR方法檢(jian)測(ce)無宿主(zhu)殘余(yu)基因組DNA；能有(you)效地(di)擴增人(ren)基(ji)因組中的單拷貝基(ji)因。
● 使用(yong)說明(ming)：
1. 冰浴中徹底(di)融化2×MasterMix，混勻后(hou)Minispin將溶液收集到管底(di)。
2. 按照下表在0.2ml PCR管中制備反應體系(xi)：
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50μl反(fan)應(ying)體系
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終濃度
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2×MasterMix
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25μl
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1×
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上(shang)游引(yin)物 10μM
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1-5μl
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0.2-0.8 μM
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下游(you)引物 10μM
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1-5μl
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0.2-0.8 μM
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模(mo)板
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× μl
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10pg-1μg
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水(shui)
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補至50μl
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3. 快甩(shuai)離心(xin)將(jiang)反應(ying)液收(shou)集到管底。
4. PCR儀如果沒有熱(re)蓋加熱(re)的話，補(bu)加25μl礦物油。
5. PCR儀上執行以(yi)下程序(xu)：
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步驟(zou)
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溫度
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時間
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循環數
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初始變性(xing)
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94℃
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5 min
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1
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變性
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94℃
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30 sec
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25-40*
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退(tui)火
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Tm-5℃*
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30 sec
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延(yan)伸(shen)
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72℃
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1 min/kb
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最后延(yan)伸(shen)
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72℃
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5 min
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1
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*注： PCR 反應(ying)條件(jian)視模(mo)板、引物等的(de)結(jie)構(gou)條件(jian)不(bu)同而各(ge)異。在實際操作(zuo)中需根據模(mo)板、目的(de)片段(duan)的(de)大(da)小、堿基序列和引物的(de)長短(duan)等具體情況(kuang)，設(she)定最佳的(de)反應(ying)條件(jian)（溫度、時間等）。
產(chan)品示例：
2×Taq PCR MasterMix擴增效果比較
M III：RTM403 DNA Marker III
lane 1：Rice 5000 bp
lane 2：Ricie 3000 bp
lane 3: Rice 600 bp
引用RTC3104 2×Taq PCR MasterMix含染料發表文章
1. [2013 IF=1.63] Preventive effect of
total glycosides from Ligustri Lucidi Fructus against nonalcoholic fatty liver
in mice
Author: Nianyun Yang, Yiwen Zhang and Jianming Guo
Product：RTC3104 2×Taq PCR MasterMix含(han)染料
Journal: Z. Naturforsch. 2015; 70(9-10)c:
237–241
Institution：Nanjing University of Chinese Medicine
Paper link： //pubmed.ncbi.nlm.nih.gov/26501160/
2. [2015 IF=1.377] Expression and
localization of guanine
nucleotide-binding protein
alpha S in
the testis and
epididymis of rams
at different developmental stages
Author: Zhen Li, Jieli
Lu, Jia Chen,
Quanhai Pang, Ruipeng
Nan, Zhiwei Zhu
Product：RTC3104 2×Taq PCR MasterMix含(han)染料
Journal: Animal
Reproduction Science. Volume
178, March 2017, Pages 31-39
Institution：Shanxi Agricultural University
Paper link：
3. [2018 IF=2.9] Integration of gene
expression profile data to screen and verify immune-related genes of chicken
erythrocytes involved in disease virus Marek’s
Author: Ali Raza Jahejo, Sayyad Ali Raza Bukhari, Fa-Jie Jia,
Sayed Haidar Abbas Raza, Mujahid Ali Shah, Nasir Rajput, Anam Ahsan, Sheng Niu,
Guan-Bao Ning, Ding Zhang, Yu-Hai Bi, Qi-Hui Wang, Ling-Xia Han, Wen-Xia Tian
Product：RTC3104 2×Taq PCR MasterMix含(han)染料(liao)
Journal: Microbial Pathogenesis. 148 (2020)
104454
Institution：Shanxi Agricultural University
Paper link：
4. [2018 IF=1.69] Study on the correlation
between BMPR1Bprotein in sheep blood and reproductive performance
Author: Xiaoyan Zhang, Liping Zhang, Weibo Sun, Xia Lang,
Jianping Wu, Caiye Zhu,Jianlei Jia, Jinpen Jin, Younfu La, David P. Casper
Product：RTC3104 2×Taq PCR MasterMix含染料(liao)
Journal: Journal of Animal Science. 2020 May
1;98(5)
Institution：Gansu Agricultural University
Paper link：
5. [2019 IF=2.6] Natural occurrence
of Alternaria mycotoxins in wheat and potential of reducing
associated risks using magnolol.
Author: Dongmei Jiang, DizheWei, Hongtao Li,LiuqingWang,Nan
Jiang,Yunchao Li, Meng Wang
Product：RTC3104 2×Taq PCR MasterMix含(han)染料
Journal: Journal of the Science
of Food and Agriculture. Volume101, Issue7,May 2021
Institution：Beijing Academy of Agriculture and
Forestry Sciences
Paper link：
6. [2022 IF=6.9] Galactinol synthase 2
infuences the metabolism of chlorophyll, carotenoids, and ethylene in tomato
fruits.
Author: Huidong Zhang, Kunpeng Zhang, Xueya Zhao, Mengxi Bi,
Yudong Liu, Shuo Wang, Yi He，Kui Ma, Mingfang Qi
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Experimental Botany Published:
15 March 2024
Institution：College of Horticulture, Shenyang
Agricultural University
Paper link：