2×Taq
PCR MasterMix
● 產品包(bao)裝：
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目錄號(hao)
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包(bao)裝
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價格
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2×MasterMix（不含染料）
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RTC3103-02
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500μl
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60元(yuan)
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RTC3103-03
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5×500μl
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240元
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2×MasterMix（含染料）
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RTC3104-02
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500μl
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60元
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RTC3104-03
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5×500μl
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240元
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注：以(yi)50μl PCR反(fan)應體系為例，每(mei)次使用25μl 2×MasterMix，500μl可做20次 50μl PCR反應。
● 儲存條件: -20℃可長期(qi)保存(cun)；4℃穩定(ding)貯存(cun)3個(ge)月。經常使用(yong)時，一(yi)旦融化后請(qing)4℃貯存(cun)，盡量避(bi)免反(fan)復凍融。
● 產品簡介：
本產品包(bao)含Taq DNA聚(ju)合酶、dNTPs、MgCl2、反(fan)應緩沖液(ye)、PCR反(fan)應的(de)(de)增(zeng)(zeng)強劑(ji)和(he)優(you)化劑(ji)以(yi)及(ji)穩定劑(ji)，濃度(du)為(wei)2×。使(shi)用(yong)(yong)時，只需(xu)補加(jia)實驗(yan)所需(xu)的(de)(de)引物和(he)模(mo)板(ban)后即可進行PCR反(fan)應，可最大限(xian)度(du)的(de)(de)減少人為(wei)誤(wu)差，具有快速(su)簡便、穩定性(xing)好等優(you)點。適用(yong)(yong)于常規PCR反(fan)應、復雜模(mo)板(ban)（如GC含(han)量高(gao)，有二級(ji)結構）的(de)(de)擴增(zeng)(zeng)以(yi)及(ji)大規模(mo)基(ji)(ji)因檢(jian)測。使(shi)用(yong)(yong)該產品得到的(de)(de)PCR擴增(zeng)(zeng)產物末(mo)端(duan)含(han)有一個A堿(jian)基(ji)(ji)，可以(yi)直接用(yong)(yong)于TA克(ke)隆。
● 質量(liang)控制：
經檢測無(wu)外源核酸酶活性；PCR方法檢測無宿主殘余(yu)基因組DNA；能有效(xiao)地擴(kuo)增人基因組中的單拷(kao)貝基因。
● 使用說(shuo)明：
1. 冰浴中徹底(di)融化2×MasterMix，混勻(yun)后Minispin將溶液收集到管底(di)。
2. 按照(zhao)下表在0.2ml PCR管中制(zhi)備反應體系：
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50μl反(fan)應體(ti)系
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終濃度
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2×MasterMix
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25μl
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1×
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上(shang)游(you)引物(wu) 10μM
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1-5μl
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0.2-0.8
μM
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下游(you)引物 10μM
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1-5μl
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0.2-0.8
μM
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模板
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× μl
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10pg-1μg
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水
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補至50μl
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3. 快(kuai)甩離心將反應(ying)液收集到(dao)管(guan)底。
4. PCR儀(yi)如果(guo)沒(mei)有熱蓋加(jia)熱的話(hua)，補(bu)加(jia)25μl礦物油(you)。
5. PCR儀上執(zhi)行以下程序：
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步驟
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溫度(du)
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時間
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循環數
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初始變(bian)性(xing)
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94℃
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5 min
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1
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變性
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94℃
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30 sec
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25-40*
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退火
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Tm-5℃*
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30 sec
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延伸
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72℃
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1 min/kb
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最后延伸
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72℃
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5 min
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1
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*注(zhu)： PCR 反應條件(jian)視模板、引物(wu)(wu)等的(de)結(jie)構條件(jian)不同而(er)各異。在實(shi)際操作中需(xu)根(gen)據模板、目的(de)片段的(de)大小、堿基序列和引物(wu)(wu)的(de)長短等具體情況，設定(ding)最佳的(de)反應條件(jian)（溫(wen)度、時間等）。
產品示(shi)例：
2×Taq PCR MasterMix擴增效果比較
M III：RTM403 DNA Marker III
lane 1：Rice 5000 bp
lane 2：Ricie 3000 bp
lane 3: Rice 600 bp
引用RTC3104 2×Taq PCR MasterMix含染料發表文章
1. [2013 IF=1.63] Preventive effect of
total glycosides from Ligustri Lucidi Fructus against nonalcoholic fatty liver
in mice
Author: Nianyun Yang, Yiwen Zhang and Jianming Guo
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Z. Naturforsch. 2015; 70(9-10)c:
237–241
Institution：Nanjing University of Chinese Medicine
Paper link： //pubmed.ncbi.nlm.nih.gov/26501160/
2. [2015 IF=1.377] Expression and
localization of guanine
nucleotide-binding protein
alpha S in
the testis and
epididymis of rams
at different developmental stages
Author: Zhen Li, ; Jieli
Lu, Jia Chen,
Quanhai Pang, Ruipeng
Nan, Zhiwei Zhu
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Animal
Reproduction Science. Volume
178, March 2017, Pages 31-39
Institution：Shanxi Agricultural University
Paper link：
3. [2018 IF=2.9] Integration of gene
expression profile data to screen and verify immune-related genes of chicken
erythrocytes involved in disease virus Marek’s
Author: Ali Raza Jahejo, Sayyad Ali Raza Bukhari, Fa-Jie Jia,
Sayed Haidar Abbas Raza, Mujahid Ali Shah, Nasir Rajput, Anam Ahsan, Sheng Niu,
Guan-Bao Ning, Ding Zhang, Yu-Hai Bi, Qi-Hui Wang, Ling-Xia Han, Wen-Xia Tian
Product：RTC3104 2×Taq PCR MasterMix含(han)染料
Journal: Microbial Pathogenesis. 148 (2020)
104454
Institution：Shanxi Agricultural University
Paper link：
4. [2018 IF=1.69] Study on the correlation
between BMPR1Bprotein in sheep blood and reproductive performance
Author: Xiaoyan Zhang, Liping Zhang, Weibo Sun, Xia Lang,
Jianping Wu, Caiye Zhu,Jianlei Jia, Jinpen Jin, Younfu La, David P. Casper
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Animal Science. 2020 May
1;98(5)
Institution：Gansu Agricultural University
Paper link：
5. [2019 IF=2.6] Natural occurrence
of Alternaria mycotoxins in wheat and potential of reducing
associated risks using magnolol.
Author: Dongmei Jiang, DizheWei, Hongtao Li,LiuqingWang,Nan
Jiang,Yunchao Li, Meng Wang
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of the Science
of Food and Agriculture. Volume101, Issue7,May 2021
Institution：Beijing Academy of Agriculture and
Forestry Sciences
Paper link：
6. [2022 IF=6.9] Galactinol synthase 2
infuences the metabolism of chlorophyll, carotenoids, and ethylene in tomato
fruits.
Author: Huidong Zhang, Kunpeng Zhang, Xueya Zhao, Mengxi Bi,
Yudong Liu, Shuo Wang, Yi He，Kui Ma, Mingfang Qi
Product：RTC3104 2×Taq PCR MasterMix含染料
Journal: Journal of Experimental Botany Published:
15 March 2024
Institution：College of Horticulture, Shenyang
Agricultural University
Paper link：