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首頁 > 核酸生物學 > 核酸電泳 > 雙鏈DNA Marker

D2000 DNA ladder(100-2000bp)試用裝

D2000 DNA ladder(100-2000bp)試用裝

產品編號:RTM415-S

產品規格:20次

相關規格(ge):
數(shu)量
價格 ¥0

D2000 DNA Ladder

 

● 產品(pin)編號(hao)及規(gui)格(ge):

    RTM415-02   ;    100T(2×250μl)      

 

● 產品簡介:

本產品是(shi)由6條帶狀雙鏈DNA條(tiao)帶組成(cheng),適用于(yu)瓊(qiong)脂糖凝膠(jiao)電泳中DNA條(tiao)帶的分析(xi)。產品中已(yi)含有1×loading buffer,可根(gen)據(ju)實驗(yan)需(xu)要,直接取2-5μl電泳(yong)(yong),使用方便,電泳(yong)(yong)圖像清晰。6條帶的大小分別(bie)為10025050075010002000bp。其中750bp條(tiao)帶為100ng/5μl,顯(xian)示加亮(liang)帶(dai)(dai),其余條帶(dai)(dai)濃(nong)度50ng/5μl


● 使用方法:

1. 2-5μl本產品加入到瓊(qiong)脂糖凝膠的加樣孔中(每1mm×1mm加樣孔加1μl,如果加樣(yang)孔寬(kuan)于

5mm,可以適當增加上樣量(liang))就行電泳(yong)。

2. 建議電泳條件(jian):凝膠濃度為1.5-2.0%,凝膠長度5-7cm,電泳電壓4-10v/cm,電(dian)泳時間15-20

分鐘(zhong)。

3. 通過EB染色后紫外燈(deng)下(xia)觀察(cha)條(tiao)帶。

注:如果(guo)使用Goldview等染料就行染色,由于靈(ling)敏度(du)比EB低(di),請酌情(qing)增加Marker的(de)上樣量。

● 注(zhu)意事項(xiang):

1. 瓊脂糖的質量對DNA的(de)電泳(yong)有很大影響,電泳(yong)時請(qing)盡量使用質量優等的(de)瓊脂糖(tang)。

 

2. 請(qing)使用新鮮配(pei)制的電泳緩沖(chong)液和新鮮配(pei)制的瓊脂(zhi)糖凝膠進行電泳,以保證Marker良好的(de)分離效(xiao)果。

使用RTM415  D2000 DNA ladder產品發表部分文章列表:

1. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415,D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link

 

2. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415,D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER,ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa2015,227 (3): 229–242

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link

 

3. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415,D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos OnePublished: December 21, 2015

InstitutionKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link

 

4. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415,D2000 DNA ladder

Author: Huan Jiang,Wenxing Long, Hui Zhang,Chengneng Mi,Tao Zhou,Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

InstitutionInstitute of Tropical Agriculture and Forestry, Hainan University

Paper link 

 

5. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415,D2000 DNA ladder

Journal: Phytotaxa. 2021,483 (2): 085–094.

Institution:University of Electronic Science and Technology of China

Paper link

 

6. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415,D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7,2021,3071-3077

InstitutionBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link

 

7. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415,D2000 DNA ladder

Author: Kevin D Hyde,Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

InstitutionSchool of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link


 
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